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oligo-nf

Simple Nextflow pipeline for a SLURM cluster. $PWD is not used to ensure user views shell scripts.

Relatedness analysis

This workflow will view chr2 from all input files. Merge into single VCF, run vcftools --relatedness2 over the merged file. Outputs a relatedness table: phi > 0.34 is possibly from the same sample. Usage, edit paths to point to own folder:

sbatch ./nextflow_vcf.nf

sbatch ./run_merge.sh

sbatch ./vcftools.sh

VEP annotations

This pipeline can be modified to run on the UT HPC to 1) install an Ensembl VEP singularity container. Modifiy paths and uncomment install-vep.sh

Run with sbatch ./install-vep.sh

Modify paths in nextflow_vep.sh to point to a folder containing .VCF files.

Run with sbatch ./nextflow_vep.sh

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Simple Nextflow pipeline for a SLURM cluster

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