DrosWAFO-py

Data analysis tool for calculating Wall Falling Behavior (WAFO) in drosophila from frame-by-frame x-y positions. Outputs a .csv file containing average Distance From Center (aDFC), which is a metric for determining WAFO.

---

Usage Guide

1. Prepare Input Files:

   • Ensure that your input files are tab-delimited .txt files containing columns for Pos. X (mm) and Pos. Y (mm).

2. Run the Program:

   • Launch the program in your Python environment.
   • Use the slider at the top to select how many flies you want to process. (Max 10)
   • Click the 'Select File #" to upload your .txt file/directory.

3. Specify Center Points:

   • For each file, input the x and y coordinates of the center point of the arena in the corresponding boxes.

4. Provide Pixel-to-MM Conversion Ratio:

   • Input the pixel-to-mm ratio in the format pixels:mm (e.g., 224:10).

5. View Results:

   • The program will display the average distance from the center in both pixels and millimeters for each file.
   • At the end of the run, the program will save the data to .csv and prompt you select the save location for the file.

---