Sample group
Once that one of the above-mentioned analysis is completed, if it was executed on multiple samples, a sample group of those samples will be automatically created. A sample group includes all the samples analyzed for that project and contains all the samples' information which is essential for the further analyses implemented in RNAdetector such as the differential expression analysis and pathway analysis (read the next sections).
However, if users want to analyze only a subgroup of a previously created sample group or merge more sample groups in a new final sample group it is possible by using the New Samples Group function available from our dashboard. To create a new sample group three steps are required
- Choose name and type. Here users can write the sample code, the analysis name, and select the samples type (Long RNAs, Small RNAs, or Circ RNAs). If users wish to create a new annotation for the samples, you have to select the De Novo Annotation option. This option will create a novel sample annotation using the provided description files (in TSV format). The sample code is used for further analysis and it identifies the samples during the analysis. Therefore, it has to be a string without any spaces (only letters, numbers, and dashes). Click Next to proceed with the next step.
- Select analysis. Here users can choose which samples will be included in this new sample group. It is possible to add not only one or more samples but also other previously created sample groups and merge them in a new final sample group. If you select previously generated sample groups to be merged, all their samples and annotations will be included in the final sample group (annotations are ignored if De Nove Annotation was selected in the previous step). Click Next to proceed with the next step.
- Upload files. Finally, here users can upload an optional sample description table (in TSV format) that can be used for sample annotation and differential expression analysis. In the end, click on the Save button to proceed with the creation of a new sample group.
