User guide introduction
RNAdetector allows users to perform several RNA-Seq data analysis through a very intuitive GUI. From the dashboard users can select:
- Small RNA-Seq analysis for the analysis of small RNA-Seq data. It can be used for identification and quantification of small ncRNAs such as miRNAs, piRNAs, tRFs, and tsRNAs.
- RNA-Seq analysis for the analysis of standard RNA-Seq data. It can be used for the identification and quantification of RNA species longer than 200 nt such as mRNAs, lncRNAs, and tUCR.
- Circular RNA analysis for identification and quantification of circRNAs.
Once one of the abovementioned analysis is performed, it is possible to execute downstream analysis such as:
- Differential expression analysis to compare the difference in the expression profile of such RNA molecules between case vs control samples
- Pathway analysis to execute a miRNA-sensitive topological pathway analysis on the results obtained by the differential expression analysis. However, currently only the differentially expressed mRNAs and miRNAs can be analyzed.
Refer to the next pages for more details.